Cultivation of Red Seaweed Kappaphycus Alvarezii (Doty)

Cultivation of sanguine Seaweed Kappaphycus Alvarezii (Doty)Cultivation of red seaweed Kappaphycus alvarezii (Doty) in deeper-sea urine of South Sulawesi, IndonesiaPetrus A. WennoAbstract. The culture of red seaweed Kappaphycus alvarezii in shallow wet is at present increased in congruity with the necessity to multiply biomass. This economic commodity is highly demand for its polysaccharide. However, an motion to expand the finish discipline is difficult to several steers. This problem whitethorn be overcome by employing some interruption commodes at different abstrusenesss. This research was carried out for 49 old age under trine different treatments, i.e., two morphological types ( parkland and embrown), one-third sign seedlings (50, deoxycytidine monophosphate and clg) and volt different wet abstrusenesss (100, 250, 400, 550 and 700 cm). The result showed signifi rear endt differences in replication and biomass among three treatments (PKeywords Kappaphycus alva rezii, deeper wet, day-after-day harvest-festival tramp, biomass, carrageenanIntroduction. The culture of red seaweed Kappaphycus alvarezii has been conducted in nigh around Indonesia waters recently since it was first introduced (Adnan and Porse 1987). Normally, the culture take place in shallow waters at a astuteness of close 10 meters, but not all waters can be utilized for culturing. Seaweed biomass obtained through longline technique in shallow waters can produce about 12 tons acre yr-1 (Dahuri 2012). The product can exit 48 tons acre yr-1 when vertical technique is deployed in deep water (Hurtado and Agbayani 2002). Deep water area can be used as a productive culture site when meet the requirements of seeds quality, the sign slant and water depth. The utilization of deep waters should be in placement to the status of coral reefs around the location as an essential requirement for obtaining higher(prenominal)(prenominal) biomass return (Chandrasekaran et al. 20 08).High biomass employment achieves through deep water culture system (Hurtado-Ponce et al. 1996) suggest that the deep water can be used for large-scale tillage. Sahoo et al. (2002) and Sahoo and Ohno (2003) suggest that water comes from deep column is very rich in nutrients and can be used alternatively as laboratory culture media and civilisation media for Kappaphycus and otherwise types of seaweed. However, the use of deep water has several constrains related to physical and chemical characteristics, cultivation equipment and p leashr surround to make it usable. As an important commodity with high market demand, cultivation of K. alvarezii requires broader area in order to meet market demand (Nurdjana 2010).thither are few studies related to return and carrageenan capacity generating in shallow waters (Hurtado et al. 2008 Naguit et al. 2009). However, there were not many studies dealt with growth and the mother of carrageenan in deeper waters. This study was aimed to ass ess the growth of K. alvarezii in deeper waters based on different strains, sign free cant overs and depths of the waters that affect growth, biomass and the take back of carrageenan. 2Material and Method. Seedlings of atomic number 19 and brown strains of K. alvarezii were obtained directly from the cultivation area in the Takalar Regency of South Sulawesi. These seeds were used after three days acclimatization. advanced seeds are the young plants with their tips still sharp and in conical wreak (Neish 2005). The seeds were then put at hanging raft (Figure 1) and monitored for 49 days for growth, biomass and carrageenan yield.Hanging rafts (Wenno 2014) were designed to replace hanging rope techniques (Hurtado et al. 2001). Each raft consists of two polyvinyl chloride pipes (L = 200 cm, = 5 cm) change with a mixture of concrete cement which served as the weigh. Both PVC pipes connected by two pieces of wood (H x W x L 5 x 7 x 400 cm) at the end to form a raft. The total ar ea of raft is 8 m2 (W x L 200 x 400 cm). Some nylon draw ( = 4 mm) with the length of 4 m were used to connect twain PVC pipes and functioning as a binding place for seaweed seeds. The quad mingled with the nearest two strings was 20 cm. The raft was hung in the water column with the help of the buoy ropes ( = 10 mm) and was displace at different depths (100, 250, 400, 550 and 700 cm). The buoy was made of Styrofoam (H x W x L 40 x 50 x 50 cm). During operation, the first raft was connected to the second the second raft was connected to the third and so on until the fifth at a maximum depth of 700 cm. The hanging rafts construction was then buttoned to the cast anchor ropes ( = 12 mm), retardation the anchor was made from flour sacks filled with sand (Figure 1).buoywater linenylon ropeshangring raftsPVC with concretecement insidesandbags anchorraft, view from aboveFigure1. Hanging rafts construction, viewed from frontage (Source Wenno 2014)Seaweed seeds were then tied to the span ropes according to tie-tie technique (Goes and Reis 2010). Seedlings from different sign heavinesss (50, 100 and 150 g) were tied to the twin knot ropes ( 1 mm). The closest distance between two nodes was 20 cm. Seeds of different strains and different initial weight (250, 500, 750 g) were placed on different rafts at different depth.Samplings were carried out for sevener consecutive weeks. Data were then used to calculate day-after-day growth appreciate (DGR) which was determined weekly using the following formula of Dawes et al. (1993) in Hurtado et al. (2001) as follows= 0 1 100Where DGR = daily growth pasture (%) = unused weight at day t0 = initial fresh weightt = time interval of measurement (7 days) 3Seaweed biomass was recorded at the end of experiment and expressed as fresh weight of seaweed per unit culture area (g.m-2), and computed with the following formula= 0 Where Y = biomass production = fresh weight atday t0 = initial fresh weightA = area of 1 m2 raft. The measurement of carrageenan contents (yield) following the formula suggested in Hayashi et al. (2007) and Hung et al. (2009)= 100Where YC = carrageenan content (%) = weight of carrageenan extract (g) = dry weight of analysed thallus (g)Three compute analysis of variance according to Zar (1999) were used to analyse the experiment information and was performed with SPSS v 21 software. Subsequent analysis with Tukeys HSD test was computed when there were pregnant differences among treatments with level of significance (PResults and Discussions. chance(a) growth rate of Kappaphycus alvarezii shows the interaction between strain and initial weight, strain and depth, as comfortably as the initial weight and depth which was highly substantive (P0.05). Further test showed that the highest daily growth rate of jet plane and brown strains was obtained at the initial weight of 100g, which tend to be the analogous (2.84%). It was related to the density of plant (Hurtado et al, 2008) , take to rapid growth in the initial weight. Daily growth rate at the initial weight of 100g was influenced by the interaction between solar radiation, temperature, nutrient and water movement (Santelices 1999), and causing absorption of nutrients faster than other initial weights. Absorption of nutrients was influenced by the density of plants (Azanza-Corrale et al. 1996).Daily growth rate of K. Alvarezii of common and brown strains tend to be the same. The highest similarity daily growth rate in green strain was achieved at the depths of 100 and 250 cm (2.55%), and the lowest one was at 700 cm depth (2.23%). The highest similarity daily growth rate in brown strain was besides achieved at the depth of 100 and 250 cm (2.83%), and the lowest one was at a depth of 700 cm (2.57%). The highest daily growth rate of green and brown strains associated with the movement of water (Santelices 1999). The movement of the water at that depth was turbulent, so reduce the thickness of water t hat is not mixed in the marge mold (Neish 2005), and the absorption of nutrients in this depth is faster. Glenn and Doty (1990) suggested that the absorption of nutrients during the fast period water between thalli is higher for ammonium at a write down depth than for nitrate at the higher depth. The absorption of ammonium by seaweed is more important than nitrate (Dy and Yap 2001 Raikar and Wafar 2006).Similarly to daily growth rate, biomass production of K. alvarezii showed the interaction between strain and initial weight, strain and depth, as well as the initial weight and depth which was highly significant (P0.05). The highest biomass production of green strain was achieved for the initial weight of 100g (10,219 g.m-2), and the lowest with the initial weight of 50g (6,709 g.m-2). The highest biomass production of brown strain was found for the initial weight of 150 g (11,450 g.m-2), and the lowest one with the initial weight of 50g (7,479 g.m-2). The highest biomass product ion achieved was related to the density of K. alvarezii thalli (Hurtado et al. 2008) that affect the circulation of nutrients. With the initial weight of 100g the biomass production of green and brown strains gained was two times higher than the initial weight of 50g. The 4biomass production of green strain with the initial weight of 100g was optimal and whitethorn not be increased above this initial weight whilst the biomass production of brown strain can be increased up to the initial weight of 150g.The highest biomass production of green strain can be obtained at a depth of 100 cm (9,172 g.m-2) and of brown strain at a depth of 250 cm (10,522 g.m-2). The similarity of the highest biomass production of both strains obtained at depths of 100 and 250 cm. The highest biomass production similarity of brown strain was achieved at 100 and 250 cm depth. This is related to the absorption of nutrients in the swallow depths which is faster than that of the higher depth (Neish 2005). Turbul ent water movement causes the thickness of the boundary layer between the water and thalli reduced and accelerated the diffusion of nutrients into thalli (Neish 2005). Biomass production of K. alvarezii was influenced by the interaction between nutrients and the movement of seawater among thalli (Santelices 1999).The yield of carrageenan in K. alvarezii shows the interaction between strain and initial weight, strain and depth, as well as the initial weight and depth and was highly significant (P0.05). The yield of carrageenan of green and brown strains was obtained with low initial weight. The yield of green strain obtained with the initial weight of 50g was higher (15.06%) than that of the brown strain (12.33%) of the same initial weight. The yield of green and brown strains was higher at the higher depths when compared to the lower depths. The highest yield of carrageenan in green strain was obtained at a depth of 550 cm (14.71%) and brown strain at the depth of 700 cm (12.18%). T he highest yield of carrageenan is the result of the interaction between depth and the initial weight at a depth of 700 cm with the initial weight of 50g (14.70%), followed by the initial weight of 100 g (13.25%) and the initial weight of 150g (11.95%). The results showed that the yield of carrageenan in both strains was not in line with the daily growth rate. It was found that the yield of carrageenan in this study was contrarily with the finding of Hurtado et al. (2008) with a long line cultivation technique, which yields an increase in line with the daily growth rate.In this study, carrageenan yield of K. alvarezii green and brown strain increases with the depth and corresponding with the increase level of carotenoid which obtained at the highest carotenoid level at a depth of 700 cm for both strains. Carotenoid acts as antenna pigments for absorbing light in the work out of photosynthesis to produce carbohydrates. However, carrageenan yield in this study is lower than the findi ngs of Hayashi et al. (2007) and Distantina et al. (2011).Conclusions. Daily growth rate of K. Alvarezii green and brown strains was influenced by the initial weight and tend to be reduced by an increasing of depth. Biomass production was also reduced by the increasing depth with the highest biomass of green strain obtained with lower initial weight, and brown strain with higher initial weight. The yield of carrageenan increased according to depth with the highest yield was observed at green strain compare to brown strain. To obtain higher biomass and carrageenan yield, cultivation should be done at a lower depth for green strain and at a rather lower to higher depth for brown strain.Acknowledgements. This study was supported by BPPS grant of DGHE from Ministry of Education and ending Affair of Republic of Indonesia. The author would like to thanks Mr. Akrim Djusdil, Chairman and Mr. genus Mus Mulyadi, Analyst of PT. Bantimurung Indah in Maros Regency of South Sulawesi for their he lp and thanks also to Daeng Bani the seawee